Studies on the conformation of human serum high-density lipoproteins HDL2 and HDL3.
نویسندگان
چکیده
منابع مشابه
Studies on Human Serum High Density Lipoproteins
Human serum apolipoprotein A-I (ape-A-I), the major protein component of the human serum high density lipoproteins, was studied in aqueous solutions of differing ionic strengths and pH by the techniques of sedimentation equilibrium ultracentrifugation and frontal analysis gel chromatography. The ultracentrifugal studies indicate that apo-A-I is a self-associating system that is dependent upon p...
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We compared the dual-precipitation method for measurement of cholesterol in high-density lipoprotein subfractions HDL2 and HDL3 (Gidez et al., J Lipid Res 1982;23:1206-33) with density-gradient ultracentrifugation in a swinging-bucket rotor (Demacker et al., Clin Chem 1983;29:656-63). The concentration of dextran sulfate 15,000 (DS) needed for optimal accuracy of the HDL2-chol and HDL3-chol val...
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High density serum lipoprotein underwent serologic and physicochemical alterations on aging during storage at 0 degrees C. for 1 month, as judged by decrease of diffusion coefficient and increase of C' fixation. Ultracentrifugation, dialysis, and high concentrations of sodium chloride did not cause these changes. A protein sedimenting at density 1.24 in the ultracentrifuge reacted with antiseru...
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The major Lipoprotein classes are heterogenous. Human serum High-Density-Lipoproteins (HDL) are separated into two major subfractions (HDL2, d=1.063-1.125 g/ml, HDL3, d=1.125-1.210 g/ml) by a simple double precipitation method. There was a strong inverse relation between HDL-Cholesterol (HDL-C) and subfractions HDL2-C and HDL3-C with atherosclerosis. This study demonstrated that HDL-C (P<0.001)...
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Persons with type 2 diabetes mellitus (T2DM) have an elevated risk of atherosclerosis. High-density lipoproteins (HDL) normally protect against cardiovascular disease (CVD), but this may be attenuated by serum amyloid A (SAA). In a case-control study of young females, blood samples were compared between subjects with T2DM (n = 42) and individuals without T2DM (n = 42). SAA and apolipoprotein AI...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1965
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.54.6.1699